Literaturdatenbank
Literaturdatenbank |
|
 |
|
Todd, E., Blair, D., Hamann, M., & Jerry, D. (2011). Twenty-nine microsatellite markers for two australian freshwater turtles, elseya albagula and emydura macquarii krefftii: development from 454-sequence data and utility in related taxa. Conservation Genetics Resources, (accepted).
Added by: Sarina Wunderlich (25 Jan 2011 10:32:11 UTC) |
| Resource type: Journal Article DOI: 10.1007/s12686-010-9377-0 BibTeX citation key: Todd2011 View all bibliographic details  |
Categories: General Keywords: Chelidae, Elseya, Elseya albagula, Elusor, Elusor macrurus, Emydura, Emydura macquarii, Genetik = genetics, Rheodytes, Rheodytes leukops, Schildkröten = turtles + tortoises Creators: Blair, Hamann, Jerry, Todd Collection: Conservation Genetics Resources |
Views: 6/975
Views index: 20% Popularity index: 5% |
| Abstract |
|
Abstract 454 shotgun sequencing was used to identify thousands of in silico microsatellite loci from two Australian freshwater turtle species (Pleurodira, Chelidae), Elseya albagula and Emydura macquarii krefftii. Twenty-nine polymorphic microsatellites were subsequently developed and tested as markers, 15 for E. m. krefftii and 14 for E. albagula. The former marker set showed greater allelic richness (mean N A = 7.7, range 3–15) than the latter (mean N A = 4.4, range 2–8). Levels of expected heterozygosity were high for both marker suites (mean H E = 0.718, range 0.177–0.875; and mean H E = 0.567, range 0.317–0.827, respectively), indicating both are suitable for studies of population genetic structure, connectivity and mating systems in these taxa. Cross-species amplification in 12 other short-necked freshwater turtle taxa highlighted the potential utility of these markers in other species, notably Emydura spp., Rheodytes leukops and Elusor macrurus.
Added by: Sarina Wunderlich |