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Engstrom, T. N., Edwards, T., Osentoski, M. F., & Myers, E. M. (2007). A compendium of pcr primers for mtdna, microsatellite, and other nuclear loci for freshwater turtles and tortoises. In B. H. Shaffer, N. N. FitzSimmons, A. Georges & A. G. J. Rhodin (Eds.), Defining Turtle Diversity: Proceedings of a Workshop on Genetics, Ethics, and Taxonomy of Freshwater Turtles and Tortoises Vol. 4, (pp. 124–141). Lunenburg: Chelonian Research Foundation. 
Added by: Sarina Wunderlich (30 Jun 2012 22:00:51 UTC)
Resource type: Book Article
BibTeX citation key: Engstrom2007
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Categories: General
Keywords: Genetik = genetics, Schildkröten = turtles + tortoises
Creators: Edwards, Engstrom, FitzSimmons, Georges, Myers, Osentoski, Rhodin, Shaffer
Publisher: Chelonian Research Foundation (Lunenburg)
Collection: Defining Turtle Diversity: Proceedings of a Workshop on Genetics, Ethics, and Taxonomy of Freshwater Turtles and Tortoises
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Abstract     
Molecular markers have proven to be a powerful tool for research on turtles. In particular, the application of the polymerase chain reaction (PCR) has increased the availability of molecular technologies while decreasing the cost. However, the cost, time, and expertise associated with developing and testing primers for a particular species can still present a significant barrier, especially to researchers less experienced with molecular methods. In this paper we provide the primer sequence, genomic location, and taxa for 202 PCR primers spanning the entire mitochondrial genome. We also report primers for 11 nuclear coding genes and introns. Finally, we provide primer sequence, amplicon size, and number of observed alleles for 181 microsatellite loci from all major clades of living turtles. We hope that this nearly comprehensive compilation of freshwater turtle and tortoise PCR primers can reduce some of the initial difficulties for beginning turtle geneticists and further facilitate research in existing labs.
Added by: Sarina Wunderlich  
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