Literaturdatenbank
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Origgi, F. C., Klein, P. A., Tucker, S. J., & Jacobson, E. R. (2003). Application of immunoperoxidase-based techniques to detect herpesvirus infection in tortoises. Journal of Veterinary Diagnostic Investigation, 15(2), 133–140.
Added by: Admin (14 Aug 2008 20:36:25 UTC) |
| Resource type: Journal Article BibTeX citation key: Origgi2003 View all bibliographic details  |
Categories: General Keywords: Schildkröten = turtles + tortoises, Testudinidae, Veterinärmedizin = veterinary medicine, Viren = viruses Creators: Jacobson, Klein, Origgi, Tucker Collection: Journal of Veterinary Diagnostic Investigation |
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| Abstract |
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Testudinidae Indirect (IIP) and direct (DIP) immunoperoxidase assays were developed for the serological and histological diagnoses of herpesvirus infection in tortoises, respectively. A mouse monoclonal antibody (MAb HL1546), specific for the heavy chain of tortoise IgY, was used as the secondary antibody in the IIP assay. Rabbit polyclonal antisera raised against 2 sucrose gradient-purified tortoise herpesvirus isolates (HV4295/7R/95 and HV1976) were used as primary antibodies for the detection of herpesvirus antigen either in infected cell cultures or in formalin-fixed, paraffin-embedded tissues. The IIP and DIP assays could detect either the presence of anti-herpesvirus antibody in the plasma of exposed tortoises or the presence of herpesvirus antigen in infected tissues, respectively. Although the IIP test complements the enzyme-linked immunosorbent assay and the serum neutralization test already available for measuring herpesvirus-specific antibody in tortoises, the DIP test is useful for the histological diagnosis of herpesvirus infection in tortoises.
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