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Veríssimo, J., Pereira, P., Teixeira, J., & Velo-Antón, G. , Optimization of microsatellite markers and genetic characterization of iberian populations of the mediterranean pond turtle (mauremys leprosa). Unpublished paper presented at XII Congresso Luso Espanhol de Herpetologia. 
Added by: Admin (06 Jan 2014 18:23:13 UTC)
Resource type: Conference Paper
BibTeX citation key: Verssimo2012
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Categories: General
Keywords: Emydidae, Genetik - genetics, Geoemydidae, Habitat - habitat, Mauremys caspica, Mauremys leprosa, Mauremys rivulata, Schildkröten - turtles + tortoises, Südwesteuropa - South-Western Europa, Trachemys scripta
Creators: Pereira, Teixeira, Velo-Antón, Veríssimo
Collection: XII Congresso Luso Espanhol de Herpetologia
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Abstract     
The Mediterranean pond turtle (Mauremys leprosa) is widespread from the Iberian Peninsula and adjacent France to northwest Africa, from western Morocco to western Libya. Despite it is considered locally abundant in many parts of its range, it is facing multiple threats such as pet trade, diseases, pathogens and the invasion of Trachemys scripta and other exotic species. The impact of these threats on the species led to an increased awareness of the lack of knowledge concerning the genetic diversity and structure of this species. The current knowledge of the evolutionary history of M. leprosa is based on sequence variation of the mitochondrial cytochrome b gene across the species range. However, the low level of genetic variation shown at mitochondrial level in terrapins precludes an accurate assessment of their evolutionary histories and often shows a lack of genetic structure within species. Here, we assessed genetic diversity patterns and population structure across Iberian populations of M. leprosa using microsatellite markers. We optimized 10 markers by cross-amplification using previously developed microsatellites in Mauremys caspica and Mauremys rivulata. We genetically characterized 11 Iberian populations covering most part of the species distribution and a set of individuals of unknown origin from a Recovery Center. Our main goals are to: 1) get insights into the evolutionary history of M. leprosa in the Iberian Peninsula; 2) characterize the genetic structure of the species; 3) identify patterns of humanmediated translocations and 4) develop a genetic tool that will assist to geographically allocate individuals from Recovery Centers and thus allowing its liberation into its origin location. Overall, by assessing the species genetic diversity and structure we can identify which are the lineages or populations more valuable for protection in the future if the species status becomes more endangered.
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