Literaturdatenbank
Literaturdatenbank |
|
 |
|
Bian, X., Zhang, L., Yang, L., Yang, P., Ullah, S., Zhang, Q., & Chen, Q. (2013). Ultrastructure of epididymal epithelium and its interaction with the sperm in the soft-shelled turtle pelodiscus sinensis. Micron, (in press, corrected proof).
Added by: Admin (06 Jan 2014 18:24:04 UTC) |
| Resource type: Journal Article BibTeX citation key: Bian2013 View all bibliographic details  |
Categories: General Keywords: Morphologie - morphology, Pelodiscus sinensis, Schildkröten - turtles + tortoises, Trionychidae Creators: Bian, Chen, Ullah, Yang, Yang, Zhang, Zhang Collection: Micron |
Views: 5/743
Views index: 19% Popularity index: 4.75% |
| Abstract |
|
Trionychidae Highlights We describe the ultrastructural characteristics of epididymis in Chinese soft-shelled turtle. Epithelium height and cell composition of different epididymal regions very varied through the reproductive cycle. The interaction between spermatozoa and epididymal epithelium present different ways. Abstract The epididymis of the soft-shelled turtle Pelodiscus sinensis was examined under light and transmission electron microscopes to determine the morphological characteristics, as well as their changes at different phases of the seasonal reproductive cycle. Three distinct regions, viz., cranial, middle and caudal were identified in the epididymis based on anatomical characteristics. The epididymal epithelium consists of five different cell types: principal, narrow, apical, clear and basal cells. Principal cells, which are the most abundant, together with basal cells are present along the entire length. Ultrastructural evidence suggests that all of the principal cells in each of the regions function in both absorption and secretion. Narrow cells and apical cells are rare and only confined to the cranial region. The clear cells, for the first time reported in the turtle epididymis, are confined to middle and caudal regions; these cells showed strong PAS-positive granulation in apical position, and secretory activity by a holocrine process, especially in the middle region. There was a significant difference in the epithelium height of all the regions between the reproductive season and the non-reproductive season. Sperm are stored in the epididymis throughout the year. Apart from the mature spermatozoa, immature spermatozoa with normal morphology are also observed. Under TEM, the immature spermatozoa showed a large amount of cytoplasm located eccentrically on the midpiece wrapped by plasma membrane, with some cytoplasm extended to the posterior of the head. Furthermore, the interactions of sperm with the epididymal epithelium were observed. Some sperm are associated with the secretory material in the lumen; other sperm are inserted into the intercellular space between the epithelial cells.
Added by: Admin |