Literaturdatenbank
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García, G., Ramos, F., Gutiérrez, R., Yañez, J., Estrada, M. S., & Hernández, L., et al. (2013). Molecular epidemiology and genetic diversity of entamoeba species in a chelonian collection. Journal of Medical Microbiology, (Published online ahead of print).
Added by: Admin (06 Jan 2014 18:24:27 UTC) |
| Resource type: Journal Article DOI: 10.1099/jmm.0.061820-0 BibTeX citation key: Garca2013 View all bibliographic details  |
Categories: General Keywords: Chrysemys picta, Einzeller - protozoa, Emydidae, Schildkröten - turtles + tortoises, Veterinärmedizin - veterinary medicine Creators: Estrada, García, Gaytán, Gutiérrez, Hernández, Martínez-Hernández, Ramos, Yañez Collection: Journal of Medical Microbiology |
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| Abstract |
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Kinosternon Chrysemys picta Veterinarian medicine has recently focused on reptiles because of the existence of captive collections in zoos and an increase in the acquisition of reptiles as pets. The Entamoeba protozoan parasite can causes amoebiasis in various animal species and humans. In reptiles, amoebiasis disease is remarkably rare in most species of chelonians, and crocodiles, and these species may serve as Entamoeba spp. carriers who transmit parasites to susceptible reptile species, such as snakes, and lizards, that can become sick and die. In this study, we identify the Entamoeba species and evaluated their diversity in a population of healthy chelonian (disease-free) by amplification and sequencing of a SSU rDNA region. Through this procedure, three Entamoeba species were identified: E. invadens in 4.76% of chelonians, E. moshkovskii in 3.96% and E. terrapinae in 50%; we did not detect mixed Entamoeba infections. Comparative analysis of the amplified region allowed us to determine the intra-species variations. The E. invadens and E. moshkovskii in this study did not show great difference with respect to the sequences reported in the GeneBank. In E. terrapinae, three different subgroups were identified (A, B and C). Even though subgroups A and C are very alike, subgroup B showed a relative great difference with respect to subgroups A and C (Fst=0.984 and Fst=1.000; 10-14% nucleotide variation by BLAST) and with respect to sequences reported in the GeneBank. These results suggest that E. terrapinae B may be in a process of speciation, or perhaps it belongs to a different linage. However, it is necessary much more work to make a statement of this kind.
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