Literaturdatenbank
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Heckers, K. O., Rüschoff, B., Stöhr, A., & Marschang, R. E. , Detection of a new herpesvirus in european pond turtles (emys orbicularis) in germany. Unpublished paper presented at 1. International Conference on Avian, Herpetological & Exotic Mammal Medicine.
Added by: Admin (06 Jan 2014 18:24:33 UTC) |
| Resource type: Conference Paper BibTeX citation key: Heckers2013 View all bibliographic details  |
Categories: General Keywords: Gopherus agassizii, Schildkröten - turtles + tortoises, Testudinidae, Veterinärmedizin - veterinary medicine, Viren - viruses Creators: Heckers, Marschang, Rüschoff, Stöhr Collection: 1. International Conference on Avian, Herpetological & Exotic Mammal Medicine |
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| Abstract |
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Within two months in early summer six turtles from a mixed collection, including four European pond turtles, and a Russian tortoise died after transfer to a newly built pond. Two of the European pond turtles were submitted for pathohistological examination. Both had necrosis of the tip of the tail with brown crusts. One of the animals also had similar lesions on the legs. An enlarged liver with multiple submiliar white foci was noted in both animals. Multiple necrotic foci with acute bleeding with no inflammatory infiltration were found in the livers. Intranuclear basophilic inclusions were found in hepatocytes associated with these lesions. Liver, intestine, lung, and kidney of each animal and spleen and skin of one animal were submitted for virological testing. All tissues were tested for the presence of viruses by isolation in cell culture (Terrapene heart cells, TH-1) and for herpes-, rana-, and adenoviral DNA by PCR. Liver, intestine, lung, and kidney from both animals and spleen of one animal were positive for herpesvirus DNA by PCR targeting a portion of the DNA polymerase gene. Sequencing of the PCR products (181 bp) showed the closest relationship (82% identitiy) to TeHV-2 from a desert tortoise (Gopherus agassizii) in the USA. This is the first detection and partial characterization of a herpesvirus in a turtle.
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