Literaturdatenbank
Literaturdatenbank |
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Papp, T. (2012). Detection and characterisation of adeno-, irido- and paramyxoviruses in reptiles. Unpublished thesis , Szent István University.
Added by: Admin (06 Jan 2014 18:25:06 UTC) |
| Resource type: Thesis/Dissertation BibTeX citation key: anon2012.15492 View all bibliographic details  |
Categories: General Keywords: Echsen - saurians, Schildkröten - turtles + tortoises, Schlangen - snakes, Stigmochelys pardalis, Testudinidae, Testudo hermanni, Veterinärmedizin - veterinary medicine, Viren - viruses Creators: Papp Publisher: Szent István University |
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| Abstract |
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Testudinidae Geochelone pardalis Testudo hermanni The first reptilian paramyxovirus (rPMV) was described in 1972 with respiratory or CNS signs. The isolated virus (Fer de Lance Virus - FDLV) is considered the type species of all rPMVs. Phylogenetic analysis of this and further squamatid PMVs justified the establishment of a new genus Ferlavirus. But subgrouping within this genus remained controversial, and prevalence of the PMVs in captive populations was not surveyed and no genetic information was available from non-squamate ferlaviruses. In our study, previously uncharacterised ferlavirus isolates from six captive snakes, three lizards and a tortoise were compared based on sequences of three genes (L, HN, U). The tortoise ferlavirus clustered as the most ancient branch in the new genus, while the other squamatid isolates separated in three groups. The established new groups “A” and “B” were in sensu lato extensions of groups from earlier reports, however, the new group “C” members (from a corn snake from Germany and a masked water snake from Hungary) were very distinctly related to any other previously described squamatid PMV. The genus characteristic U gene was identified in all squamatid ferlaviruses but could not be detected in the tortoise isolate. In a PCR survey of PMV infection of snakes, fifteen different sequence variants were identified either belonging to “group A” or “group B” squamatid ferlaviruses. The observed prevalence (27.5%) was surprisingly high, and concurrent infection with more than one PMV type was recorded in different organs of the same snake and/or in different snakes originating from the same populations. Similarly, in a leopard tortoise (Geochelone pardalis) with severe respiratory distress, 3 different squamatid ferlavirus types were identified in four different organs. These findings along with a recent report on a non-ferlavirus snake PMV from Australia underline the importance of further PCR and serological PMV surveys in both captive and wild reptile populations.
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