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Perpiñán, D., Costa, T., Bargalló, F., & Martínez-Silvestre, A. , Correlation between gonad histology and endoscopic dex determination in juvenile read-eared turtles (trachemys scripta elegans). Unpublished paper presented at 1. International Conference on Avian, Herpetological & Exotic Mammal Medicine. 
Added by: Admin (06 Jan 2014 18:25:08 UTC)
Resource type: Conference Paper
BibTeX citation key: Perpin2013
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Categories: General
Keywords: Emydidae, Schildkröten - turtles + tortoises, Trachemys scripta, Untersuchungsmethoden - examination method, Veterinärmedizin - veterinary medicine
Creators: Bargalló, Costa, Martínez-Silvestre, Perpiñán
Collection: 1. International Conference on Avian, Herpetological & Exotic Mammal Medicine
Views: 4/683
Views index: 18%
Popularity index: 4.5%
Abstract     
Coelioscopy has been proven to be a valuable technique to determine the sex of juvenile chelonians. However, there has been controversy regarding the proper way to identify testes and ovaries. Controversy has arisen from the lack of studies correlating histology of the gonad with the results of the endoscopic examination. In this study, we assessed two methods of sex determination in 15 juvenile (93-164 g) Trachemys scripta: coelioscopy with direct gonad visualization, and cloacoscopy with phallus/clitoris visualization. We later compared these with post-mortem examination and histology of the gonads. The veterinarian performing cloacoscopy (AMS) did not know the results from the veterinarian performing coelioscopy (DP), and vice-versa. In addition, the pathologist (TC) did not know the results from coelioscopy and cloacoscopy. Histology correlated 100% with the results from coelioscopies, but only 53.33% of the sexing results made by cloacoscopy were accurate. Cloacoscopy diagnosed 100% of males as females and 38.46% of females as males. Testicle in juvenile turtles was a round to oval, smooth, yellow organ, with blood vessels on its surface and attached to the dorsal structures by short but evident ligaments on both poles. Ovaries were long, irregular, semi-transparent organs where developing, round, white to yellow follicles could be seen developing inside the organ. Follicles were separated from each other and did not fill completely the parenchyma of the organ.
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