Literaturdatenbank
Literaturdatenbank |
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Chen, Z., Zheng, J., & Jiang, Y. (1999). A new iridovirus isolated from soft-shelled turtle. Virus Research, 63(1/2), 147–151.
Added by: Admin (14 Aug 2008 22:46:40 UTC) |
| Resource type: Journal Article BibTeX citation key: Chen1999b View all bibliographic details  |
Categories: General Keywords: Pelodiscus, Pelodiscus sinensis, Schildkröten = turtles + tortoises, Trionychidae, Trionyx, Viren = viruses Creators: Chen, Jiang, Zheng Collection: Virus Research |
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| Abstract |
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A virus was isolated from soft-shelled turtle (Trionyx sinensis) with 'red neck disease' on a farm in Shenzhen China. The virus multiplied and caused a cytopathogenic effect (CPE) at 15-30 deg C in grass carp ovary (CO), fathead minnow (FHM), grass carp kidney (CK) and blue-gill fry-2 (BF-2) cells. The optimum conditions for replication was in CO cells at 25-30 deg C. The virus was sensitive to chloroform, acid (pH 3) or alkaline (pH 10) conditions, and heating at 56 deg C for 30 min. Treatment with 5-iodo-2-deoxyuridine (IUDR) inhibited viral replication, indicating a DNA genome. Electron microscopy of infected CO culture fluid showed spherical particles, 120- 160 nm in diameter. Ultra-thin sections showed numerous hexagonal viral particles in the cytoplasm and nucleus of cells typical of an iridovirus. This virus was moderately virulent for turtles in infection tests. The name soft-shelled turtle iridovirus (STIV) is suggested.
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