Literaturdatenbank
Literaturdatenbank |
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Fleming, A. A., & Crews, D. (2001). Estradiol and incubation temperature modulate regulation of steroidogenic factor 1 in the developing gonad of the red-eared slider turtle. Endocrinology, 142(4), 1403–1411.
Added by: Admin (14 Aug 2008 22:46:42 UTC) |
| Resource type: Journal Article BibTeX citation key: Fleming2001 View all bibliographic details  |
Categories: General Keywords: Emydidae, Schildkröten = turtles + tortoises, Trachemys, Trachemys scripta, Zeitigung = incubation Creators: Crews, Fleming Collection: Endocrinology |
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| Abstract |
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Trachemys scripta Red-eared slider turtles are genetically bipotential for sex determination, with incubation temperature of the egg determining gonadal sex. At higher incubation temperatures, females are produced, possibly due to increased biosynthesis of oestrogen Exogenous oestrogen causes the formation of ovaries, and prevention of oestrogen biosynthesis results in the development of testes. In mammals, steroidogenic factor 1 (SF- 1) regulates most genes required for oestrogen biosynthesis from cholesterol. In mammals as well as red-eared slider turtles, SF-1 is differentially expressed in males and females during gonadogenesis. To examine a possible role for SF-1 in temperature-dependent sex determination, we assayed its expression in red-eared slider turtles after treatments that alter sex development during gonadogenesis of the wild-type organism. We examined gonadal SF-1 expression in embryos (1) incubating at three different temperatures, (2) after treating eggs with oestrogen at a male-producing temperature and (3) after inhibition of oestrogen biosynthesis at a female-producing temperature. Our findings suggest that both temperature and oestrogen lie upstream of SF-1 in a sex-determining regulatory hierarchy in red-eared slider turtles and that oestrogen directly or indirectly modulates the regulation of SF-1 expression.
Added by: Admin |