Literaturdatenbank
Literaturdatenbank |
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Marschang, R. E., Becher, P., Posthaus, H., Wild, P., Thiel, H. J., & Müller-Doblies, U., et al. (1999). Isolation and characterization of an iridovirus from hermann's tortoises (testudo hermanni). Archives of Virology, 144(10), 1909–1922.
Added by: Admin (14 Aug 2008 22:46:49 UTC) |
| Resource type: Journal Article BibTeX citation key: Marschang1999b View all bibliographic details  |
Categories: General Keywords: Schildkröten = turtles + tortoises, Testudinidae, Testudo, Testudo hermanni, Viren = viruses Creators: Bacciarini, Becher, Kaleta, Marschang, Müller-Doblies, Posthaus, Thiel, Wild Collection: Archives of Virology |
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| Abstract |
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A virus isolated from tissues of 2 diseased Hermann's tortoises (Testudo hermanni) was characterized as an iridovirus based on the presence of inclusion bodies in the cytoplasm of infected cells, sensitivity to chloroform, inhibition of virus replication by 5-iodo-2'-deoxyuridine and the size and icosahedral morphology of viral particles. The virus was able to replicate in several reptilian, avian and mammalian cell lines at 28 deg C, but not at 37 deg C. Restriction enzyme analysis showed resistance of the viral DNA to digestion with HpaII due to methylation of the internal cytosine at CCGG sequences. Part of the genomic region encoding the major capsid protein was amplified by PCR and subjected to sequence analysis. Comparative analysis of the obtained nucleotide sequence showed that the isolate is closely related to frog virus 3, the type species of the genus Ranavirus.
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