Literaturdatenbank
Literaturdatenbank |
|
 |
|
Une, Y., Murakami, M., Uemura, K., Fujitani, H., Ishibashi, T., & Nomura, Y. (2000). Polymerase chain reaction (pcr) for the detection of herpesvirus in tortoises. Journal of Veterinary Medical Science, 62(8), 905–907.
Added by: Admin (14 Aug 2008 22:46:57 UTC) |
| Resource type: Journal Article BibTeX citation key: Une2000 View all bibliographic details  |
Categories: General Keywords: Chelonoidis, Chelonoidis carbonaria, Geochelone, Malacochersus, Malacochersus tornieri, Schildkröten = turtles + tortoises, Stigmochelys, Stigmochelys pardalis, Testudinidae, Testudo, Testudo horsfieldii, Veterinärmedizin = veterinary medicine, Viren = viruses Creators: Fujitani, Ishibashi, Murakami, Nomura, Uemura, Une Collection: Journal of Veterinary Medical Science |
Views: 3/624
Views index: 10% Popularity index: 2.5% |
| Abstract |
|
A polymerase chain reaction (PCR)-based method using a herpesvirus consensus primer was assessed for the identification of herpesviral infections in tortoises (Malacochersus tornieri, Testudo horsfieldii, Geochelone pardalis and G. carbonaria). A single band of about 230 bp was detected in PCR products from 2 out of 20 swabs taken from the oral cavity, 3 out of 3 paraffin-embedded tissue sections from the liver (2 cases) and oral mucosa (1 case), and 1 out of 2 fresh tissue samples from the oral mucosa. Nucleotide sequencing of these PCR products indicated that the herpesvirus present in these tortoises might belong to the alphaherpesvirinae. PCR using swabs and biopsy tissues was a sensitive and highly specific method for the diagnosis of herpesviral infections in tortoises. The nucleotide sequence reported in this paper has been deposited at GenBank under accession number AB027762 Testudinidae
Added by: Admin |