Literaturdatenbank
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Chen, Z., Zheng, J., & Jiang, Y. (1998). Iridovirus isolated from a soft-shelled turtle with "red neck disease". Chinese Journal of Veterinary Science, 18(2), 135–139.
Added by: Admin (17 Aug 2008 17:23:45 UTC) |
| Resource type: Journal Article BibTeX citation key: Chen1998a View all bibliographic details  |
Categories: General Keywords: Pelodiscus, Pelodiscus sinensis, Schildkröten = turtles + tortoises, Trionychidae, Trionyx, Veterinärmedizin = veterinary medicine, Viren = viruses Creators: Chen, Jiang, Zheng Collection: Chinese Journal of Veterinary Science |
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| Abstract |
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A virus, isolated from a soft-shelled turtle (Trionyx sinensis) with "red neck disease" on a farm in Shenzhen city, multiplied and caused a CPE at 15- to 30 deg C in carp ovary (CO), fathead minnow and carp kidney cells. Optimum replication was at 25-30 deg C in CO cells. An agarose-covered CO cell monolayer formed plaques, 1-2 mm in diameter 2-3 days after infection. The virus was not stable to chloroform treatment, pH 3 or pH 9; treatment with 5-iodo-2-deoxyuridine inhibited its replication, indicating the presence of a DNA genome. By electron microscopy, the infected CO culture fluid revealed spherical particles measuring 120 to 160 nm in diameter. Observation of thin sections showed numerous hexagonal viral particles in the cytoplasm and nuclei of the cells. Infection tests showed this virus was of medium virulence to turtles. There was no serological relationship between this virus and infectious pancreatic necrosis virus, grass carp haemorrhagic disease reovirus, and chum salmon virus. The name suggested for this virus is soft-shelled turtle iridovirus.
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