Literaturdatenbank
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Zhang, Q., Li, Z., & Hu, J. (2000). Serological detection for the trionyx sinensis virus. Acta Hydrobiologica Sinica, 24(4), 356–361.
Added by: Admin (17 Aug 2008 17:24:13 UTC) |
| Resource type: Journal Article BibTeX citation key: Zhang2000 View all bibliographic details  |
Categories: General Keywords: Blut = blood, Pelodiscus, Pelodiscus sinensis, Schildkröten = turtles + tortoises, Trionychidae, Trionyx, Veterinärmedizin = veterinary medicine, Viren = viruses Creators: Hu, Li, Zhang Collection: Acta Hydrobiologica Sinica |
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| Abstract |
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Trionyx sinensis virus (TSV) was isolated from Chinese soft-shelled turtles, where it causes a lethal disease. Antibodies to the virus (TSV-Ab) were produced from rabbits raised to TSV and purified from infected EPC cells. The neutralization titre of TSV-Ab was 1:20. The TSV-Ab was applied to immunodiffusion and indirect ELISA for detection of the viral pathogen. The samples tested included homogenates of healthy and diseased turtle tissues, TSV infected cell extracts, purified TSV, and other viruses from aquatic animals. Immunodiffusion and ELISA results using the TSV-Ab indicated that infectious pancreatic necrosis virus (IPNV), grass carp reovirus (GCV), viral haemorrhagic septicaemia virus (VHSV), carp pox virus, monodon baculovirus (MBV) and the homogenate of health turtle tissues were seronegative, while the infected cell extracts and the homogenate of diseased turtle tissues were seropositive. Thus, the immunodiffusion and the ELISA test were easy and sensitive methods for detecting TSV in the turtles.
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