Literaturdatenbank
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Roosenburg, W. M., Elskus, A. A., Ford, D. K., & Palmer, B. D. , Survivorship, growth, and bioassays of terrapin hatchlings incubated on oil contaminated nesting areas. Paper presented at SETAC 22nd Annual Meeting.
Added by: Admin (17 Aug 2008 18:54:57 UTC) |
| Resource type: Proceedings Article BibTeX citation key: Roosenburg2001 View all bibliographic details  |
Categories: General Keywords: Emydidae, Habitat = habitat, Malaclemys, Malaclemys terrapin, Nordamerika = North America, Schildkröten = turtles + tortoises, Toxikologie = toxicology, Zeitigung = incubation Creators: Elskus, Ford, Palmer, Roosenburg Collection: SETAC 22nd Annual Meeting |
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| Abstract |
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ABSTRACT- We conducted an in situ nesting experiment to investigate the effects of an oil spill on nest survivorship and hatchling growth of the diamondback terrapin, Malaclemys terrapin. An oil spill on April 7th 2000 in the Patuxent River in Chesapeake Bay left many of the nesting beaches covered with #2 fuel oil and #6 crude oil. We collected eggs from natural nests, randomized the eggs, and constructed 9 nests on a contaminated beach and 10 nests on a control beach. Nests had 11 or 12 eggs and a temperature logger to monitor nest temperatures. Thermal regimes of the two sites were very similar with no significant difference in average nest temperature. Hatching success was significantly lower at the contaminated beach than at the control beach, 76 vs 98%. Ten hatchlings from each group were sacrificed, and plasma and livers collected. Plasma from hatchlings was analyzed for the induction of vitellogenin using western blots with diamondback terrapin-specific anti-vitellogenin antibodies. However, no vitellogenin was detected. Livers were assayed for catalytic activity of CYP1A, an enzyme biomarker of organic pollutant exposure. We found no differences in CYP1A activity levels between the two groups as measured by ethoxyresorufin o-deethylase (EROD) (0.85 ± 0.30 (oiled) and 1.10 ± 0.31 (ref) pmol/min/mg). At four months, post-hatching growth was significantly reduced in hatchlings from contaminated beaches. Hatchlings from the contaminated beach lost weight (-0.60 ± 0.24 g) while hatchlings from the clean beach gained weight (0.14 ± 0.20 g). Additionally, shell structure and rigidity appeared to be compromised in the individuals from the contaminated beaches. The growth experiment is still in progress and we will present additional results as our study progresses.
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