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Gumpenberger, C. (1996). Steroidhormongehalt im kot von griechischen und maurischen landschildkröten im jahresgang und kontrolle der ovarien mit ultraschalluntersuchungen. Unpublished thesis thesis, Veterinärmedizinische Universität, Wien. 
Added by: Admin (23 Aug 2008 15:32:59 UTC)
Resource type: Thesis/Dissertation
BibTeX citation key: Gumpenberger1996
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Categories: General
Keywords: Fortpflanzung = reproduction, Physiologie = physiology, Schildkröten = turtles + tortoises, Testudinidae, Testudo, Testudo graeca, Testudo hermanni, Untersuchungsmethoden = examination method, Veterinärmedizin = veterinary medicine
Creators: Gumpenberger
Publisher: Veterinärmedizinische Universität (Wien)
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Views index: 9%
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Abstract     
Testudinidae Steroid concentrations were measured in faecal samples of Testudo hermanni and T. graeca after methanolic extraction and enzymatic hydrolysis using enzymeimunoassays for estrone, epiandrosterone and corticosterone. The ultrasound imaging of follicles and eggs was performed through the inguinal shell opening using a 7,5 MHz transducer. In females median values of 6 nmol estrone / kg faeces, 250 nmol epiandrosterone / kg faeces and 70 nmol corticosterone / kg faeces were measured. In males 3 nmol estrone / kg faeces, 800 nmol epiandrosterone / kg faeces and 110 nmol corticosterone / kg faeces were detected. All three steroids showed positive significant correlation's. In most females maximum corticosterone levels were measured during the first half of the animals' activity period, whereas maximum estrone and epiandrosterone levels could be found in the second half. In most male tortoises maximum corticosterone and estrone concentrations were measured between the end of April and the end of July whereas maximum epiandrosterone concentrations were found from August until the middle of October. The androgen / estrogen ratio in faecal samples can be used for sex determination in adult T. hermanni and T. graeca. Follicular growth was examined sonographically and correlated with elevated steroid levels. However, no definitive correlation's between fecal steroid data and ovulation and nesting could be found. At the moment ultrasonography is the most economic and most effective method of monitoring reproductive cycles in tortoises
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