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Kuchling, G., Skolek-Winnisch, R., & Bamberg, E. (1981). Histochemical and biochemical investigation on the annual cycle of testis, epididymis, and plasma testosterone of the tortoise, testudo hermanni hermanni gmelin. General and Comparative Endocrinology, 44(2), 194–201. 
Added by: Admin (24 Aug 2008 20:00:21 UTC)
Resource type: Journal Article
BibTeX citation key: Kuchling1981b
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Categories: General
Keywords: Histologie = histology, Physiologie = physiology, Schildkröten = turtles + tortoises, Testudinidae, Testudo, Testudo hermanni
Creators: Bamberg, Kuchling, Skolek-Winnisch
Collection: General and Comparative Endocrinology
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Abstract     
Testudinidae Abstract Males of the tortoise Testudo h. hermanni of a natural population in Montenegro, Yugoslavia, and captive ones in an outside area in Vienna, Austria, were used for a comparative investigation. Throughout the year testis and epididymis were examined histologically and the activity of ?-N-acetylglucosaminidase in these organs was determined biochemically and histochemically. The activity of this enzyme increased in the testis and epididymis with the appearance of differentiated spermatozoa during summer. In the epithelium of the ductuli epididymides there were segments with very high and others with very low activity of this enzyme at that time. In the testis only moderate activity of 3?-hydroxysteroid dehydrogenase was found from January to June in the Leydig cells and at any time of the year in the Sertoli cells. The concentration of testosterone in blood plasma was maximal during summer, the main period of spermiogenesis, minimal during winter, and between these levels in spring, during the main mating period. The high concentration of plasma testosterone in summer may have been necessary for the activation of ?-N-acetylglucosaminidase in the ductuli epididymides at the time of the passage of spermatozoa.
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