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Verly, C. (2006). Does multiple paternity increase with female body size in the common map turtle (graptemys geographica)? Unpublished thesis B.Sc. Honours Degree, University of Ottawa, Ottawa. 
Added by: Admin (14 Aug 2008 20:33:48 UTC)   Last edited by: Beate Pfau (01 Jan 2011 17:22:28 UTC)
Resource type: Thesis/Dissertation
BibTeX citation key: Verly2006a
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Categories: General
Keywords: Emydidae, Fortpflanzung = reproduction, Genetik = genetics, Graptemys, Graptemys geographica, Habitat = habitat, Nordamerika = North America, Schildkröten = turtles + tortoises
Creators: Verly
Publisher: University of Ottawa (Ottawa)
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Abstract     
Graptemys geographica
In the common map turtle, females are much larger than males. In general, larger
females can produce more and/or bigger offspring. It has also been shown that larger
offspring survive better. Therefore, larger female map turtles should be more attractive
to males. If males incur non-trivial costs of mating, such as missed opportunities or
sperm limitation, they should mate preferentially with larger females. Accordingly,
multiple paternity should be more common in larger females. To test this prediction, we
captured 34 gravid females spanning the full size range of reproductive females and
induced egg laying with oxytocin. We then collected blood samples from 338 hatchlings
for paternity analyses. The number of sires was deducted from the paternal alleles at 3
microsatellite loci. Due to laboratory setbacks, only eight of the 34 clutches were
analyzed. A third paternal allele was found only once, for one hatchling, at one locus.
The fact that no strong evidence of multiple paternity was found is inconclusive. Low
sample size, sub sampling the clutches and the low variability of the three microsatellite
loci reduce the ability to detect multiples sires in a clutch. The question of multiple
paternity augmenting with female body size in the common map turtle is left unanswered,
but might soon come as the molecular laboratory work is almost sorted out: DNA
extraction and PCRs are troubleshooted, and fragment analysis is soon to follow.
Added by: Admin  Last edited by: Beate Pfau
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