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Rivera, S., Diaz-Figueroa, O., Frasca, S., Garner, M. M., Gregory, C. R., & Innis, C. J., et al. , Medical management of a group of confiscated indotestudo forstenii. Paper presented at Turtle Survival Alliance 2007 Annual Meeting. 
Added by: Admin (13 Dec 2008 22:23:53 UTC)
Resource type: Proceedings Article
BibTeX citation key: Rivera2007
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Categories: General
Keywords: Bakterien = bacteria, Einzeller = protozoa, Indotestudo, Indotestudo forstenii, Schildkröten = turtles + tortoises, Testudinidae, Veterinärmedizin = veterinary medicine, Viren = viruses
Creators: Diaz-Figueroa, Frasca, Garner, Gregory, Innis, Marlar, McManamon, Nyaoke, Raphael, Risatti, Rivera, Wellehan
Collection: Turtle Survival Alliance 2007 Annual Meeting
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Abstract     
Testudinidae Indotestudo forstenii (Forsten’s tortoise) inhabits the islands of Sulawesi and Halmahera in Indonesia and is currently listed in CITES appendix II. A group of Indotestudo forstenii was obtained by the Turtle Survival Alliance and was subsequently placed in facilities where veterinary care was readily available. A large percentage of the tortoises were in poor health. Clinical signs included anorexia, lethargy, mucosal ulcerations and palatine erosions in the oral cavity, nasal and ocular discharge, and diarrhea. The initial diagnostic tests included fecal testing for parasites, CBC and plasma biochemistries, mycoplasma serology, and PCR testing for intranuclear coccidia and chelonian herpesvirus. Tissue samples from animals that died were submitted for histopathology. Samples from the cloaca and various organs were submitted for bacterial culture and sensitivity. Treatment included the administration of antibiotics, antiparasitic medications, parenteral fluids, and nutritional support. Fecal testing results, gross necropsy, and histopathologic findings revealed severe intestinal and hepatic amoebiasis. The histopathologic evaluation also showed severe damage to multiple organs and intranuclear inclusion bodies consistent with a systemic viral infection. Aeromonas hydrophila and Escherichia coli were cultured from multiple organs. The mycoplasma serology and PCR results for intranuclear coccidia and chelonian herpesvirus were negative. Polymerase chain reaction testing of tissues and choanal swabs from affected animals were positive for an adenovirus. Sequencing data revealed an adenovirus that has not been described in reptiles. Currently, there is ongoing work to further characterize this novel adenovirus. This demonstrates the importance of thorough diagnostic work ups in tortoises of unknown origin that are showing high morbidity and mortality rates. It also emphasizes the importance of strict quarantine guidelines.
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