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Baker, P. J., Costanzo, J. P., & Lee, R. E. (2007). Oxidative stress and antioxidant capacity of a terrestrially hibernating hatchling turtle. Journal of Comparative Physiology B. Biochemical, Systems, and Environmental Physiology, 177(8), 875–883. 
Added by: Sarina Wunderlich (22 Dec 2008 23:45:05 UTC)   Last edited by: Beate Pfau (29 Jan 2012 16:10:36 UTC)
Resource type: Journal Article
DOI: 10.1007/s00360-007-0185-0
BibTeX citation key: Baker2007a
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Categories: General
Keywords: Chrysemys, Chrysemys picta, Emydidae, Schildkröten = turtles + tortoises, Verhalten = ethology, Zeitigung = incubation
Creators: Baker, Costanzo, Lee
Collection: Journal of Comparative Physiology B. Biochemical, Systems, and Environmental Physiology
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Hatchlings of the painted turtle, Chrysemys picta, hibernate terrestrially and can survive subfreezing temperatures by supercooling or by tolerating the freezing of their tissues. Whether supercooled or frozen, an ischemic hypoxia develops because tissue perfusion is limited by low temperature and/or freezing. Oxidative stress can occur if hatchlings lack sufficient antioxidant defenses to minimize or prevent damage by reactive oxygen species. We examined the antioxidant capacity and indices of oxidative damage in hatchling C. picta following survivable, 48 h bouts of supercooling (-6 degrees C), freezing (-2.5 degrees C), or hypoxia (4 degrees C). Samples of plasma, brain, and liver were collected after a 24 h period of recovery (4 degrees C) and assayed for Trolox-equivalent antioxidant capacity (TEAC), thiobarbituric acid reactive substances (TBARS), and carbonyl proteins. Antioxidant capacity did not vary among treatments in any of the tissues studied. We found a significant increase in TBARS in plasma, but not in the brain or liver, of frozen/thawed hatchlings as compared to untreated controls. No changes were found in the concentration of TBARS or carbonyl proteins in supercooled or hypoxia-exposed hatchlings. Our results suggest that hatchling C. picta have a well-developed antioxidant defense system that minimizes oxidative damage during hibernation.

Keywords Chrysemys picta - Cold hardiness - Overwintering - Freeze tolerance - Supercooling - Hypoxia - Oxidative damage
Added by: Sarina Wunderlich  Last edited by: Beate Pfau
Added by: Sarina Wunderlich  Last edited by: Beate Pfau
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