Literaturdatenbank
Literaturdatenbank |
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Pedall, I., Schäfer, H., Fritz, U., & Wink, M. (2008). Isolation of microsatellite markers in the emys orbicularis complex and development of multiplex pcr amplification. Conservation Genetics, 10(3), 725–727.
Added by: Admin (14 Aug 2008 20:34:34 UTC) Last edited by: Beate Pfau (03 Oct 2009 10:32:51 UTC) |
| Resource type: Journal Article DOI: 10.1007/s10592-008-9631-0 BibTeX citation key: Pedall2008a View all bibliographic details  |
Categories: General Keywords: Emydidae, Emys, Emys orbicularis, Emys trinacris, Genetik = genetics, Schildkröten = turtles + tortoises, Systematik = taxonomy Creators: Fritz, Pedall, Schäfer, Wink Collection: Conservation Genetics |
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| Abstract |
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Abstract We report the development of 15 new microsatellite markers for Emys orbicularis and Emys trinacris. A survey of 20 individuals showed that all loci are highly polymorphic with 3–14 alleles per locus. Additionally, 22 Glyptemys muhlenbergii primers were checked for cross-species amplification, with 14 being amplified successfully and polymorphic (2–14 alleles). A set of eight markers was selected and combined into two multiplex PCRs. Levels of genetic diversity were assessed in 648 individuals covering the complete distribution area. The number of alleles ranged from 13 to 24 and observed heterozygosity varied between 0.515 and 0.852.
Added by: Admin Last edited by: Beate Pfau |