Literaturdatenbank
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Hagerty, B. E., Peacock, M. M., Kirchoff, V. S., & Tracy, C. R. (2008). Polymorphic microsatellite markers for the mojave desert tortoise, gopherus agassizii. Molecular Ecology Resources, 8(5), 1149–1151.
Added by: Admin (22 Feb 2009 11:56:16 UTC) |
| Resource type: Journal Article BibTeX citation key: Hagerty2008 View all bibliographic details  |
Categories: General Keywords: Genetik = genetics, Gopherus, Gopherus agassizii, Schildkröten = turtles + tortoises, Testudinidae Creators: Hagerty, Kirchoff, Peacock, Tracy Collection: Molecular Ecology Resources |
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| Abstract |
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Testudinidae We describe primers and polymerase chain reaction (PCR) conditions to amplify 14 tri- and tetranucleotide microsatellite loci for the Mojave desert tortoise (Gopherus agassizii). Across three populations (87 individuals) located in the Mojave Desert, USA, the markers yielded a range of four to 33 alleles and an average observed heterozygosity of 0.733 (range 0.433 to 0.933). We neither detected linkage disequilibrium between any pair of loci nor did we find a consistent pattern of deviation from Hardy–Weinberg equilibrium. These microsatellites are designed for PCR multiplexing, and provide higher throughput capacity to aid in conservation genetics studies for this threatened species.
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