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Alleman, A. R., Jacobson, E. R., & Raskin, R. E. (1992). Morphologic and cytochemical characteristics of blood cells from the desert tortoise (gopherus agassizii). American Journal of Veterinary Research, 53, 1645–1651. 
Added by: Sarina Wunderlich (24 May 2009 21:32:04 UTC)
Resource type: Journal Article
BibTeX citation key: Alleman1992
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Categories: General
Keywords: Blut = blood, Gopherus, Gopherus agassizii, Schildkröten = turtles + tortoises, Testudinidae, Veterinärmedizin = veterinary medicine
Creators: Alleman, Jacobson, Raskin
Collection: American Journal of Veterinary Research
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Abstract     
Testudinidae Morphologic and cytochemical staining characteristics of erythrocytes, leukocytes, and thrombocytes of the desert tortoise (Gopherus agassizii) were evaluated, using blood smears prepared from 23 healthy tortoises of Kern County, Calif. Special emphasis was placed on differentiating features of the various leukocytes and thrombocytes. A variety of cytochemical stains, including benzidine peroxidase, Sudan black B, chloroacetate esterase, alpha-naphthyl butyrate esterase, acid phosphatase, leukocyte alkaline phosphatase, periodic acid-Schiff, and toluidine blue were used. Heterophils had a characteristic, large, focal area of positive staining with chloroacetate esterase, alpha-naphthyl butyrate esterase, and acid phosphatase. Eosinophils stained diffusely positive with benzidine peroxidase, allowing differentiation of this leukocyte from heterophils. Thrombocytes stained focally positive with periodic acid-Schiff, allowing differentiation of these cells from lymphocytes, which stained uniformly negative. An intracytoplasmic body, commonly observed within erythrocytes, was considered ultrastructurally to represent a degenerate organelle.
Added by: Sarina Wunderlich  
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