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Metin, K., Türkozan, O., Kargin, F., Basimoglu, Y. K., Taşkavak, E., & Koca, S. (2006). Blood cell morphology and plasma biochemistry of the captive european pond turtle emys orbicularis. Acta Veterinaria Brunensis, 75(1), 49–55. 
Added by: Sarina Wunderlich (24 May 2009 21:32:38 UTC)
Resource type: Journal Article
BibTeX citation key: Metin2006
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Categories: General
Keywords: Blut = blood, Emydidae, Emys, Emys orbicularis, Haltung = husbandry, Schildkröten = turtles + tortoises, Veterinärmedizin = veterinary medicine
Creators: Basimoglu, Kargin, Koca, Metin, Taşkavak, Türkozan
Collection: Acta Veterinaria Brunensis
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The morphological characteristics of peripheral blood cells,micronucleated erythrocytes counts and plasma biochemistry profile were examined in ten healthy captive European pond turtles Emys orbicularis.Blood samples were obtained from the caudal vein.The Wright staining method was used for the classification of the blood cells.Mature erythrocytes of captive Emys orbicularis were nucleated ellipsoidal cells (21.7 ±1.27 µm ×13.2 ±1.12 µm)with pink cytoplasm.The nucleus (6.9 ±0.78 µm ×5.4 ±0.65 µm)was centrally located and stained dark purple.Seven different types of blood cells were determined:erythrocytes,thrombocytes,monocytes,eosinophils,basophils, lymphocytes and heterophils.The micronucleated erythrocyte (MNE)values were 0.0016 and 0.003 for the males and females,respectively.The MNE results were presented as the mean frequency of micronucleated erythrocytes per 1000 cells per animal. The mean plasma concentrations in the total of specimens were as follows:total protein (25 g/L), albumin (7.2 g/L),globulin (17.8 g/L),glucose (2.91 mmol/L),calcium (2.32 mmol/L), phosphorus (1.55 mmol/L),creatinine (46.85 µmol/L),urea (10.93 mmol/L),triglycerides (0.44 mmol/L),cholesterol (1.48 mmol/L),sodium (125.76 mmol/L),potassium (3.98 mmol/L), chloride (93.94 mmol/L),iron (13.34 µmol/L)and activities of aspartate aminotransferase (2.14 µkat/L),alanine aminotransferase (0.15 µkat/L),gamma glutamyl transpeptidase (2.15 U/L), amylase (8.09 µkat/L),lactate dehydrogenase (19.93 µkat/L).We found sex-dependent differences only in ALT value in the blood biochemical profile for healthy Emys orbicularis . We suggest that the biochemical profile described in the present study may be used as a standard profile for healthy Emys orbicularis kept in captivity.
Added by: Sarina Wunderlich  
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