Literaturdatenbank
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Ramsey, M. E., & Crews, D. (2007). Adrenal-kidney-gonad complex measurements may not predict gonad-specific changes in gene expression patterns during temperature-dependent sex determination in the red-eared slider turtle (trachemys scripta elegans). Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 307A(8), 463–470.
Added by: Sarina Wunderlich (24 May 2009 21:41:26 UTC) |
| Resource type: Journal Article BibTeX citation key: Ramsey2007b View all bibliographic details  |
Categories: General Keywords: Emydidae, Schildkröten = turtles + tortoises, Trachemys, Trachemys scripta, Zeitigung = incubation Creators: Crews, Ramsey Collection: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
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| Abstract |
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Many turtles, including the red-eared slider turtle (Trachemys scripta elegans) have temperature-dependent sex determination in which gonadal sex is determined by temperature during the middle third of incubation. The gonad develops as part of a heterogenous tissue complex that comprises the developing adrenal, kidney, and gonad (AKG complex). Owing to the difficulty in excising the gonad from the adjacent tissues, the AKG complex is often used as tissue source in assays examining gene expression in the developing gonad. However, the gonad is a relatively small component of the AKG, and gene expression in the adrenal-kidney (AK) compartment may interfere with the detection of gonad-specific changes in gene expression, particularly during early key phases of gonadal development and sex determination. In this study, we examine transcript levels as measured by quantitative real-time polymerase chain reaction for five genes important in slider turtle sex determination and differentiation (AR, ER, ER, aromatase, and Sf1) in AKG, AK, and isolated gonad tissues. In all cases, gonad-specific gene expression patterns were attenuated in AKG versus gonad tissue. All five genes were expressed in the AK in addition to the gonad at all stages/temperatures. Inclusion of the AK compartment masked important changes in gonadal gene expression. In addition, AK and gonad expression patterns are not additive, and gonadal gene expression cannot be predicted from intact AKG measurements.
Added by: Sarina Wunderlich |