Literaturdatenbank
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Buenten, D. (2008). Distribution of the iridovirus in free-ranging eastern box turtles at brookhaven national laboratory. Unpublished thesis , Brookhaven National Laboratory, Upton NY.
Added by: Sarina Wunderlich (28 Feb 2010 12:07:07 UTC) |
| Resource type: Thesis/Dissertation BibTeX citation key: Buenten2008 View all bibliographic details  |
Categories: General Keywords: Emydidae, Habitat = habitat, Nordamerika = North America, Schildkröten = turtles + tortoises, Terrapene, Terrapene carolina, Veterinärmedizin = veterinary medicine, Viren = viruses Creators: Buenten Publisher: Brookhaven National Laboratory (Upton NY) |
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| Abstract |
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Terrapene carolina carolina The Iridovirus is a family of pathogenic disease that affects reptiles, fish, and amphibians worldwide. Currently, there are four documented genera: The Chlorirdovirus and Iridovirus (which infects invertebrates) and the Lymphocystivirus and Ranavirus (which infects cold-blooded vertebrates). A recent outbreak of this Ranavirus had been detected on Brookhaven National Lab (BNL) property among the Eastern Box Turtle population. The symptoms of the Ranavirus in Eastern Box Turtles include conjunctivitis, lethargy, oral and nasal discharge, and death. Since New York State considers this species a Special Concern, a survey was needed to track and record the size and effect of this outbreak. A group of oral and cloacal samples of Eastern Box Turtles were collected on site for DNA. These DNA samples were removed from the swabs using the Dneasy tissue kit procedure. The samples were run through Polymerase Chain Reaction twice to enhance the DNA quantity, and then placed into an electrophoresis gel. Using Geographic Information Systems (GIS), the location of all turtles found was plotted so the distribution of the virus could be seen across BNL. The results came back inconclusive for this experiment. The majority of the testing came back negative, however there was reasonable doubt as to whether the outcome was reliable. Also, there was not enough consistency in the GIS plotting to be reasonably sure of the Ranavirus’ movement.
Added by: Sarina Wunderlich |