Literaturdatenbank
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Jiang, Y., Nie, L. W., Huang, Z. F., Jing, W. X., Wang, L., Liu, L., & Dai, X. T. (2011). Comparison of complete mitochondrial dna control regions among five asian freshwater turtle species and their phylogenetic relationships. Genetics and molecular research : GMR, 10(3), 1545–1557.
Added by: Sarina Wunderlich (28 Aug 2011 21:15:12 UTC) |
| Resource type: Journal Article DOI: 10.4238/vol10-3gmr1205 BibTeX citation key: Jiang2011 View all bibliographic details  |
Categories: General Keywords: Genetik = genetics, Geoemydidae, Mauremys, Mauremys annamensis, Mauremys japonica, Mauremys mutica, Mauremys reevesii, Mauremys rivulata, Mauremys sinensis, Melanochelys, Melanochelys tricarinata, Schildkröten = turtles + tortoises, Systematik = taxonomy Creators: Dai, Huang, Jiang, Jing, Liu, Nie, Wang Collection: Genetics and molecular research : GMR |
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| Abstract |
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ABSTRACT. The complete mitochondrial DNA (mtDNA) control regions (CR), cytochrome b (Cyt b), NADH dehydrogenase subunit 4 (ND4) and cytochrome coxidase subunit I (CO I) genes of four Asian freshwater turtles, Mauremys japonica, Ocadia sinensis, M. mutica, and Annamemys annamensis, were sequenced using universal PCR and long-PCR techniques. Combined with CR sequences of Chinemys reevesii, the composition and structure of CR of the five species were compared and analyzed. Three functional domains (TAS, CD and CSB) in CR and their conserved sequences (TAS, CSB-F, CSB-1, CSB-2, and CSB-3) were identified based on sequence similarity to those of other turtles. At the 3' end of CSB, six type motifs of variable number of tandem repeats (VNTRs) of five species were recognized, in which the TTATATTA motif may be the VNTR motif of the ancestral species of these five turtles. Comparison of nucleotide divergences among Cyt b, ND4, CO I, and CR of 11 turtle species using transitions + transversions and transversions-only methods supported the conclusion that CR evolved 2.6- to 5.7-fold faster than the other mtDNA genes. After excluding VNTRs of CR, molecular phylogenetic trees were constructed with maximum parsimony, maximum likelihood and Bayesian inference methods. The results supported an expanded clade of Mauremys, which included species formerly in Ocadia, Chinemys, Mauremys, and Annamemys; this was also reflected in the results of VNTR analysis.
Added by: Sarina Wunderlich |