Literaturdatenbank
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Tada, N., Saka, M., Ueda, Y., Hoshi, H., Uemura, T., & Kamata, Y. (2004). Comparative analyses of serum vitellogenin levels in male and female reeves' pond turtles ( chinemys reevesii) by an immunological assay. Journal of Comparative Physiology B. Biochemical, Systems, and Environmental Physiology, 174(1), 13–20.
Added by: Admin (14 Aug 2008 20:36:28 UTC) |
| Resource type: Journal Article BibTeX citation key: Tada2004 View all bibliographic details  |
Categories: General Keywords: Blut = blood, Emydidae, Geoemydidae, Mauremys, Mauremys japonica, Mauremys reevesii, Schildkröten = turtles + tortoises, Trachemys, Trachemys scripta, Veterinärmedizin = veterinary medicine Creators: Hoshi, Kamata, Saka, Tada, Ueda, Uemura Collection: Journal of Comparative Physiology B. Biochemical, Systems, and Environmental Physiology |
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| Abstract |
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A quantitative immunological method was developed for measuring serum vitellogenin levels of Reeves' pond turtles ( Chinemys reevesii) to investigate the effects of endocrine disruptors on the freshwater ecosystem. Vitellogenin was induced by injecting estradiol-17beta into C. reevesii turtles (adult females, juvenile females, and males) and was purified from the turtle serum by EDTA-MgCl(2) precipitation followed by gel filtration. Using a polyclonal antibody raised against C. reevesii vitellogenin, an enzyme-linked immunosorbant assay was established. The detectable range, recovery of vitellogenin, and coefficient of variation in this assay were 0.0040-1.0 microg.ml(-1), 85.3-109% and 3.4-11.5%, respectively. This assay was also applicable for measurement of the concentrations of vitellogenins from other species, Japanese pond turtles ( Mauremys japonica) and red-eared turtles ( Trachemys scripta). The serum vitellogenin concentration of 131 C. reevesii turtles captured at a Japanese local river was measured by the assay. In females, vitellogenin ranged from 0.10 microg.ml(-1) to 15,000 microg.ml(-1) with two peaks, 0.10-1.0 microg.ml(-1) (juveniles) and 1,000-10,000 microg.ml(-1) (adults). However, in males, it ranged from 0.10 microg.ml(-1) to 0.60 microg.ml(-1), showing one peak, 0.10-0.20 microg.ml(-1). Therefore, if relatively high concentrations of vitellogenin are detected in males or juvenile females, it is suggested that they would have been exposed to xenobiotic estrogens.
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