Literaturdatenbank
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Hausmann, J. C., Wack, A. N., Allender, M. C., Cranfield, M. R., Murphy, K. J., & Barrett, K., et al. , Experimental challenge study of ranavirus infection in previously infected eastern box turtles (terrapene carolina carolina) to assess immunity (abstract). Unpublished paper presented at 2013 Box Turtle Conservation Workshop.
Added by: Admin (06 Jan 2014 18:24:33 UTC) |
| Resource type: Conference Paper BibTeX citation key: Hausmann2013 View all bibliographic details  |
Categories: General Keywords: Emydidae, Schildkröten - turtles + tortoises, Terrapene carolina, Veterinärmedizin - veterinary medicine, Viren - viruses Creators: Allender, Barrett, Bronson, Cranfield, Hausmann, Murphy, Romero, Wack, Wellehan, Zink Collection: 2013 Box Turtle Conservation Workshop |
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| Abstract |
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The Maryland Zoo in Baltimore lost 13 of 27 captive Eastern box turtles (Terrapene carolina carolina) to an outbreak of Ranavirus (100% homology to 531 bp segment of FV3 MCP) during the summer of 2011. To assess survival and shedding post-infection, an experimental challenge study was performed, in which the surviving, previously infected turtles were re-infected with the outbreak strain of Ranavirus that was harvested in terrapene heart cell lines (TH-1). Seven turtles were inoculated with a predetermined dose of infectious virus IM and four control turtles were injected with an equal volume of saline IM. The turtles were monitored for 9 weeks with blood and oral swabs collected for PCR and antibody testing. During that time only one of the seven (14.3%) inoculated turtles and none of the controls (0%) died; there was no significant difference in survival. All clinical signs seen in the inoculated turtles, except for the turtle that died, were very mild (lethargy, weight loss, oral ulcers, sublingual swelling, skin excoriations, ocular discharge and periocular swelling) when compared to the severe clinical signs shown by these turtles during the previous outbreak. The inoculated turtle that died showed intracytoplasmic inclusion bodies in the kidney, lungs, pancreas, liver, and vas deferens; vasculitis in the spleen, pancreas, lungs, and liver; nephritis; pnuemonia; esophagitis; hepatitis; and enteritis. Five of the surviving ten turtles were euthanized at the end of the study for histopathology and PCR testing. The remaining five turtles began normal brumation.
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