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Péricard, J.-M., & Blahak, S. , Occurrence of mycoplasma, chlamydia and herpes virus on land tortoises with respiratory or occular diseases in south of france. Unpublished paper presented at 1. International Conference on Avian, Herpetological & Exotic Mammal Medicine. 
Added by: Admin (06 Jan 2014 18:25:07 UTC)
Resource type: Conference Paper
BibTeX citation key: Pricard2013
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Categories: General
Keywords: Schildkröten - turtles + tortoises, Stigmochelys pardalis, Testudinidae, Testudo graeca, Testudo hermanni, Untersuchungsmethoden - examination method, Veterinärmedizin - veterinary medicine
Creators: Blahak, Péricard
Collection: 1. International Conference on Avian, Herpetological & Exotic Mammal Medicine
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Abstract     
Testudinidae 34 land tortoises presented for respiratory or ocular diseases were sampled for PCR looking for mycoplasma, Chlamydiaceae, and herpesviruses in the same sample. Herpesvirus was found only once, in a tracheobronchial lavage of a Testudo graeca with pneumonia. Mycoplasma was found 29 times: once in the eye of a Testudo hermanni with conjunctivitis, once in a tracheobronchial lavage of a Testudo graeca with deep respiratory signs, 27 times in choanal and nasal swabs of 26 Testudo graeca and 1 Geochelone pardalis with upper respiratory tract disease, including 3 Testudo graeca that also had conjunctivitis or blepharitis. Chlamydiaceae spp.were found in 7 of the Testudo graeca with upper respiratory tract disease, 6 of them also positive for mycoplasma, and 1 only for Chlamydiaceae spp. Only 3 tortoises were negative for the 3 tests, 2 Testudo graeca which received an antibiotic treatment shortly before sampling, and 1 Geochelone pardalis which is also believed to have been previously treated. In 15 tortoises without any previous antibiotic treatment, the prevalence of mycoplasma was 15/15 = 100%, Chlamydiaceae spp 5/15 = 33.3%, and herpesvirus 0/15 = 0%. This prevalence decreased for mycoplasma and Chlamydiaceae spp depending on how recently they had been treated with antibiotics active against these bacteria. The Chlamydiaceae spp. detected need to be characterized more thoroughly in future; PCR products from the detection of mycoplasma and herpesvirus will be sequenced to place them more exactly in their families.
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