Literaturdatenbank
Literaturdatenbank |
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Elsevier, S. M., & Pieau, C. (1988). Purification and partial characterization of protamines from the european pond turtle, emys orbicularis. Journal of Experimental Zoology, 246(1), 33–41.
Added by: Admin (22 Feb 2009 11:56:12 UTC) |
| Resource type: Journal Article BibTeX citation key: Elsevier1988 View all bibliographic details  |
Categories: General Keywords: Emydidae, Emys, Emys orbicularis, Genetik = genetics, Schildkröten = turtles + tortoises Creators: Elsevier, Pieau Collection: Journal of Experimental Zoology |
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| Abstract |
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Basic chromosomal proteins were purified from spermatozoa of the European pond turtle, Emys orbicularis. They were partially characterized and classified as type 1 protamines according to the definition of Bloch ( Genetics suppl., 61:93-111; In: Handbook of Genetics. R.C. King, ed. Plenum Press, New York, Vol. 5, pp. 139-167). Contrary to a previous report (Kharchenko et al., Biochemistry , 45:1239-1246), these proteins could also be isolated from Emys testis, but only during certain periods of the male seasonal reproductive cycle. The proteins migrated during gel electrophoresis similarly to the protamines that have been observed for lizards and snakes, members of a different reptile order (Kasinsky et al., J. Exp. Zool., 203:109-126). These results extend the observation that protamine type is conserved among reptile species and provide a partial characterization of the conserved proteins. They support the conclusion that an important step in the evolution of vertebrate protamines occurs with reptiles. They draw into question, however, hypotheses that have been offered to explain the mechanism bringing about this protein
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